4WR7
Crystal structure of human carbonic anhydrase isozyme I with 2,3,5,6-Tetrafluoro-4-(propylthio)benzenesulfonamide.
Summary for 4WR7
| Entry DOI | 10.2210/pdb4wr7/pdb |
| Descriptor | Carbonic anhydrase 1, ZINC ION, 2,3,5,6-tetrafluoro-4-(propylsulfanyl)benzenesulfonamide, ... (7 entities in total) |
| Functional Keywords | drug design, benzenesulfonamide, metal-binding, lyase-lyase inhibitor complex, lyase |
| Biological source | Homo sapiens (Human) |
| Cellular location | Cytoplasm: P00915 |
| Total number of polymer chains | 2 |
| Total formula weight | 58755.97 |
| Authors | Manakova, E.,Smirnov, A.,Grazulis, S. (deposition date: 2014-10-23, release date: 2015-07-01, Last modification date: 2024-01-10) |
| Primary citation | Zubriene, A.,Smirnoviene, J.,Smirnov, A.,Morkunaite, V.,Michailoviene, V.,Jachno, J.,Juozapaitiene, V.,Norvaisas, P.,Manakova, E.,Grazulis, S.,Matulis, D. Intrinsic thermodynamics of 4-substituted-2,3,5,6-tetrafluorobenzenesulfonamide binding to carbonic anhydrases by isothermal titration calorimetry. Biophys.Chem., 205:51-65, 2015 Cited by PubMed Abstract: Para substituted tetrafluorobenzenesulfonamides bind to carbonic anhydrases (CAs) extremely tightly and exhibit some of the strongest known protein-small ligand interactions, reaching an intrinsic affinity of 2 pM as determined by displacement isothermal titration calorimetry (ITC). The enthalpy and entropy of binding to five CA isoforms were measured by ITC in two buffers of different protonation enthalpies. The pKa values of compound sulfonamide groups were measured potentiometrically and spectrophotometrically, and enthalpies of protonation were measured by ITC in order to evaluate the proton linkage contributions to the observed binding thermodynamics. Intrinsic means the affinity of a sulfonamide anion for the Zn bound water form of CAs. Fluorination of the benzene ring significantly enhanced the observed affinities as it increased the fraction of deprotonated ligand while having little impact on intrinsic affinities. Intrinsic enthalpy contributions to the binding affinity were dominant over entropy and were more exothermic for CA I than for other CA isoforms. Thermodynamic measurements together with the X-ray crystallographic structures of protein-ligand complexes enabled analysis of structure-activity relationships in this enzyme ligand system. PubMed: 26079542DOI: 10.1016/j.bpc.2015.05.009 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.5 Å) |
Structure validation
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