4WOB

Proteinase-K Pre-Surface Acoustic Wave

4WOB の概要

• エントリーDOI: 10.2210/pdb4wob/pdb
• 関連するPDBエントリー: 4WO6 4WO9 4WOA 4WOC
• 分子名称: Proteinase K, SULFATE ION (3 entities in total)
• 機能のキーワード: surface acoustic wave, nanocrystals, crystal manipulation, serial crystallography, acoustic tweezers, hydrolase
• 由来する生物種: Parengyodontium album (Tritirachium album)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29054.85

構造登録者

French, J.B. (登録日: 2014-10-15, 公開日: 2015-02-18, 最終更新日: 2023-12-27)

主引用文献

Guo, F.,Zhou, W.,Li, P.,Mao, Z.,Yennawar, N.H.,French, J.B.,Huang, T.J.
Precise Manipulation and Patterning of Protein Crystals for Macromolecular Crystallography Using Surface Acoustic Waves.
Small, 11:2733-2737, 2015

PubMed Abstract: Advances in modern X-ray sources and detector technology have made it possible for crystallographers to collect usable data on crystals of only a few micrometers or less in size. Despite these developments, sample handling techniques have significantly lagged behind and often prevent the full realization of current beamline capabilities. In order to address this shortcoming, a surface acoustic wave-based method for manipulating and patterning crystals is developed. This method, which does not damage the fragile protein crystals, can precisely manipulate and pattern micrometer and submicrometer-sized crystals for data collection and screening. The technique is robust, inexpensive, and easy to implement. This method not only promises to significantly increase efficiency and throughput of both conventional and serial crystallography experiments, but will also make it possible to collect data on samples that were previously intractable.

PubMed: 25641793
DOI: 10.1002/smll.201403262

実験手法

X-RAY DIFFRACTION (1.9 Å)