4WFK

Crystal structure of PET-degrading cutinase Cut190 S226P mutant in Ca(2+)-bound state at 2.35 angstrom resolution

Summary for 4WFK

• Entry DOI: 10.2210/pdb4wfk/pdb
• Related: 4WFI 4WFJ
• Descriptor: Cutinase, CALCIUM ION, CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: cutinase, polyesterase, alpha/beta-hydrolase fold, hydrolase
• Biological source: Saccharomonospora viridis
• Total number of polymer chains: 1
• Total formula weight: 30363.45

Authors

Miyakawa, T.,Mizushima, H.,Ohtsuka, J.,Oda, M.,Kawai, F.,Tanokura, M. (deposition date: 2014-09-15, release date: 2014-12-24, Last modification date: 2020-01-29)

• Primary citation: Miyakawa, T.,Mizushima, H.,Ohtsuka, J.,Oda, M.,Kawai, F.,Tanokura, M.; Structural basis for the Ca(2+)-enhanced thermostability and activity of PET-degrading cutinase-like enzyme from Saccharomonospora viridis AHK190.; Appl.Microbiol.Biotechnol., 99:4297-4307, 2015; PubMed: 25492421; DOI: 10.1007/s00253-014-6272-8

Experimental method

X-RAY DIFFRACTION (2.35 Å)