4WC5

Structure of tRNA-processing enzyme complex 3

4WC5 の概要

• エントリーDOI: 10.2210/pdb4wc5/pdb
• 関連するPDBエントリー: 1VFG 4WB1 4WB2 4WB3 4WB4 4WB6 4WB7 4WBY 4WBZ 4WC0
• 分子名称: Poly A polymerase, RNA (74-MER), CYTIDINE-5'-TRIPHOSPHATE (3 entities in total)
• 機能のキーワード: rna nucleotidyltransferase, cca-adding enzyme, a-adding enzyme, transferase-rna complex, transferase/rna
• 由来する生物種: Aquifex aeolicus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 70884.04

構造登録者

Yamashita, S.,Tomita, K. (登録日: 2014-09-04, 公開日: 2015-04-15, 最終更新日: 2023-11-08)

主引用文献

Yamashita, S.,Martinez, A.,Tomita, K.
Measurement of Acceptor-T Psi C Helix Length of tRNA for Terminal A76-Addition by A-Adding Enzyme.
Structure, 23:830-842, 2015

PubMed Abstract: The 3'-terminal CCA (C74C75A76-3') of tRNA is required for protein synthesis. In Aquifex aeolicus, the CCA-3' is synthesized by CC-adding and A-adding enzymes, although in most organisms, CCA is synthesized by a single CCA-adding enzyme. The mechanisms by which the A-adding enzyme adds only A76, but not C74C75, onto tRNA remained elusive. The complex structures of the enzyme with various tRNAs revealed the presence of a single tRNA binding site on the enzyme, with the enzyme measuring the acceptor-TΨC helix length of tRNA. The 3'-C75 of tRNA lacking A76 can reach the active site and the size and shape of the nucleotide binding pocket at the insertion stage are suitable for ATP. The 3'-C74 of tRNA lacking C75A76 cannot reach the active site, although CTP or ATP can bind the active pocket. Thus, the A-adding enzyme adds only A76, but not C74C75, onto tRNA.

PubMed: 25914059
DOI: 10.1016/j.str.2015.03.013

実験手法

X-RAY DIFFRACTION (3.41 Å)