4WC5

Structure of tRNA-processing enzyme complex 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2014-06-18
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	58.870, 111.270, 127.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.824 - 3.410
R-factor	0.2691
Rwork	0.267
R-free	0.30860
Starting model (for MR)	4wc2
RMSD bond length	0.002
RMSD bond angle	0.531
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		3.490
High resolution limit [Å]	3.410	15.230	3.410
Rmerge	0.167	0.028	2.299
Rmeas	0.181	0.031	2.478
Total number of observations	86262
Number of reflections	11953	91	867
<I/σ(I)>	11.14	49.73	1.01
Completeness [%]	99.1	52.3	98.3
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	isopropanol, potassium chloride, magnesium chloride, sodium cacodylate