4WC5
Structure of tRNA-processing enzyme complex 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-06-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.870, 111.270, 127.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.824 - 3.410 |
| R-factor | 0.2691 |
| Rwork | 0.267 |
| R-free | 0.30860 |
| Starting model (for MR) | 4wc2 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.531 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.490 | |
| High resolution limit [Å] | 3.410 | 15.230 | 3.410 |
| Rmerge | 0.167 | 0.028 | 2.299 |
| Rmeas | 0.181 | 0.031 | 2.478 |
| Total number of observations | 86262 | ||
| Number of reflections | 11953 | 91 | 867 |
| <I/σ(I)> | 11.14 | 49.73 | 1.01 |
| Completeness [%] | 99.1 | 52.3 | 98.3 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | isopropanol, potassium chloride, magnesium chloride, sodium cacodylate |
