4WBG

Crystal structure of class C beta-lactamase Mox-1 covalently complexed with aztorenam

4WBG の概要

• エントリーDOI: 10.2210/pdb4wbg/pdb
• 関連するPDBエントリー: 3W8K
• 分子名称: Beta-lactamase, 2-({[(1Z)-1-(2-amino-1,3-thiazol-4-yl)-2-oxo-2-{[(2S,3S)-1-oxo-3-(sulfoamino)butan-2-yl]amino}ethylidene]amino}oxy)-2-methylpropanoic acid, ZINC ION, ... (5 entities in total)
• 機能のキーワード: beta-lactamase, aztreonam, acyl-intermediate, hydrolase
• 由来する生物種: Klebsiella pneumoniae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 41662.39

構造登録者

Oguri, T.,Shimizu-ibuka, A.,Ishii, Y. (登録日: 2014-09-03, 公開日: 2015-07-01, 最終更新日: 2024-10-23)

主引用文献

Oguri, T.,Ishii, Y.,Shimizu-Ibuka, A.
Conformational Change Observed in the Active Site of Class C beta-Lactamase MOX-1 upon Binding to Aztreonam
Antimicrob.Agents Chemother., 59:5069-5072, 2015

PubMed Abstract: We solved the crystal structure of the class C β-lactamase MOX-1 complexed with the inhibitor aztreonam at 1.9Å resolution. The main-chain oxygen of Ser315 interacts with the amide nitrogen of aztreonam. Surprisingly, compared to that in the structure of free MOX-1, this main-chain carboxyl changes its position significantly upon binding to aztreonam. This result indicates that the interaction between MOX-1 and β-lactams can be accompanied by conformational changes in the B3 β-strand main chain.

PubMed: 26055361
DOI: 10.1128/AAC.04428-14

実験手法

X-RAY DIFFRACTION (1.9 Å)