4WAS

STRUCTURE OF THE ETR1P/NADP/CROTONYL-COA COMPLEX

4WAS の概要

• エントリーDOI: 10.2210/pdb4was/pdb
• 関連するPDBエントリー: 4W99
• 分子名称: Enoyl-[acyl-carrier-protein] reductase [NADPH, B-specific] 1, mitochondrial, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, CROTONYL COENZYME A, ... (4 entities in total)
• 機能のキーワード: mitochondrial fatty acid synthesis, oxidoreductase
• 由来する生物種: Candida tropicalis (Yeast)
• 細胞内の位置: Mitochondrion : Q8WZM3
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 123403.24

構造登録者

Quade, N.,Voegeli, B.,Rosenthal, R.,Capitani, G.,Erb, T.J. (登録日: 2014-08-31, 公開日: 2015-03-18, 最終更新日: 2024-01-10)

主引用文献

Rosenthal, R.G.,Vogeli, B.,Quade, N.,Capitani, G.,Kiefer, P.,Vorholt, J.A.,Ebert, M.O.,Erb, T.J.
The use of ene adducts to study and engineer enoyl-thioester reductases.
Nat.Chem.Biol., 11:398-400, 2015

PubMed Abstract: An improved understanding of enzymes' catalytic proficiency and stereoselectivity would further enable applications in chemistry, biocatalysis and industrial biotechnology. We use a chemical probe to dissect individual catalytic steps of enoyl-thioester reductases (Etrs), validating an active site tyrosine as the cryptic proton donor and explaining how it had eluded definitive identification. This information enabled the rational redesign of Etr, yielding mutants that create products with inverted stereochemistry at wild type-like turnover frequency.

PubMed: 25867044
DOI: 10.1038/nchembio.1794

実験手法

X-RAY DIFFRACTION (1.7 Å)