4W2G

Crystal structure of the Thermus thermophilus 70S ribosome in complex with pactamycin (soaked), mRNA and three deacylated tRNAs in the A, P and E sites

This is a non-PDB format compatible entry.

Summary for 4W2G

• Entry DOI: 10.2210/pdb4w2g/pdb
• Descriptor: 16S Ribosomal RNA, 30S Ribosomal Protein S10, 30S Ribosomal Protein S11, ... (61 entities in total)
• Functional Keywords: pactamycin, antibiotic, 70s ribosome, inhibition of translation, initiation inhibitor, 30s subunit with pactamycin (soaked), mrna, deacylated a-, p-, and e-site trnas of the 1st 70s ribosome in the asu, functional complex of bacterial 70s ribosome with antibiotic pactamycin (compact conformation), 50s subunit of the 1st 70s ribosome in the asu, ribosome-antibiotic complex, ribosome/antibiotic
• Biological source: Escherichia coli; More
• Total number of polymer chains: 112
• Total formula weight: 4554628.56

Authors

Polikanov, Y.S.,Osterman, I.A.,Szal, T.,Tashlitsky, V.N.,Serebryakova, M.V.,Kusochek, P.,Bulkley, D.,Malanicheva, I.A.,Efimenko, T.A.,Efremenkova, O.V.,Konevega, A.L.,Shaw, K.J.,Bogdanov, A.A.,Rodnina, M.V.,Dontsova, O.A.,Mankin, A.S.,Steitz, T.A.,Sergiev, P.V. (deposition date: 2014-09-12, release date: 2014-10-15, Last modification date: 2023-12-27)

• Primary citation: Polikanov, Y.S.,Osterman, I.A.,Szal, T.,Tashlitsky, V.N.,Serebryakova, M.V.,Kusochek, P.,Bulkley, D.,Malanicheva, I.A.,Efimenko, T.A.,Efremenkova, O.V.,Konevega, A.L.,Shaw, K.J.,Bogdanov, A.A.,Rodnina, M.V.,Dontsova, O.A.,Mankin, A.S.,Steitz, T.A.,Sergiev, P.V.; Amicoumacin a inhibits translation by stabilizing mRNA interaction with the ribosome.; Mol.Cell, 56:531-540, 2014; PubMed: 25306919; DOI: 10.1016/j.molcel.2014.09.020

Experimental method

X-RAY DIFFRACTION (2.55 Å)