4V9B

Crystal Structure of the 70S ribosome with tigecycline.

Summary for 4V9B

Entry DOI	10.2210/pdb4v9b/pdb
Descriptor	16S ribosomal RNA, 30S RIBOSOMAL PROTEIN S10, 30S RIBOSOMAL PROTEIN S11, ... (56 entities in total)
Functional Keywords	rna, ribosome, trna, translation, mrna, ribosome-antibiotic complex, ribosome/antibiotic
Biological source	Thermus thermophilus More
Total number of polymer chains	108
Total formula weight	4461376.45
Authors	Jenner, L.,Yusupov, M.,Yusupova, G. (deposition date: 2012-07-18, release date: 2014-07-09, Last modification date: 2024-11-06)
Primary citation	Jenner, L.,Starosta, A.L.,Terry, D.S.,Mikolajka, A.,Filonava, L.,Yusupov, M.,Blanchard, S.C.,Wilson, D.N.,Yusupova, G. Structural basis for potent inhibitory activity of the antibiotic tigecycline during protein synthesis. Proc.Natl.Acad.Sci.USA, 110:3812-3816, 2013 Cited by PubMed Abstract: Here we present an X-ray crystallography structure of the clinically relevant tigecycline antibiotic bound to the 70S ribosome. Our structural and biochemical analysis indicate that the enhanced potency of tigecycline results from a stacking interaction with nucleobase C1054 within the decoding site of the ribosome. Single-molecule fluorescence resonance energy transfer studies reveal that, during decoding, tigecycline inhibits the initial codon recognition step of tRNA accommodation and prevents rescue by the tetracycline-resistance protein TetM. PubMed: 23431179 DOI: 10.1073/pnas.1216691110 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.1 Å)

Structure validation