4V9A

Crystal Structure of the 70S ribosome with tetracycline.

これはPDB形式変換不可エントリーです。

4V9A の概要

• エントリーDOI: 10.2210/pdb4v9a/pdb
• 分子名称: 16S ribosomal RNA, 30S RIBOSOMAL PROTEIN S10, 30S RIBOSOMAL PROTEIN S11, ... (56 entities in total)
• 機能のキーワード: rna, ribosome, trna, translation, mrna, ribosome-antibiotic complex, ribosome/antibiotic
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 106
• 化学式量合計: 4410193.87

構造登録者

Jenner, L.,Yusupov, M.,Yusupova, G. (登録日: 2012-07-18, 公開日: 2014-07-09, 最終更新日: 2024-11-20)

主引用文献

Jenner, L.,Starosta, A.L.,Terry, D.S.,Mikolajka, A.,Filonava, L.,Yusupov, M.,Blanchard, S.C.,Wilson, D.N.,Yusupova, G.
Structural basis for potent inhibitory activity of the antibiotic tigecycline during protein synthesis.
Proc.Natl.Acad.Sci.USA, 110:3812-3816, 2013

PubMed Abstract: Here we present an X-ray crystallography structure of the clinically relevant tigecycline antibiotic bound to the 70S ribosome. Our structural and biochemical analysis indicate that the enhanced potency of tigecycline results from a stacking interaction with nucleobase C1054 within the decoding site of the ribosome. Single-molecule fluorescence resonance energy transfer studies reveal that, during decoding, tigecycline inhibits the initial codon recognition step of tRNA accommodation and prevents rescue by the tetracycline-resistance protein TetM.

PubMed: 23431179
DOI: 10.1073/pnas.1216691110

実験手法

X-RAY DIFFRACTION (3.2999 Å)