4V91
Kluyveromyces lactis 80S ribosome in complex with CrPV-IRES
This is a non-PDB format compatible entry.
Summary for 4V91
| Entry DOI | 10.2210/pdb4v91/pdb |
| Related | 4CUX 4CUY |
| EMDB information | 2599 |
| Descriptor | 25S RRNA, EL8, UL6, ... (45 entities in total) |
| Functional Keywords | translation, initiation, ribosome, ires |
| Biological source | KLUYVEROMYCES LACTIS More |
| Total number of polymer chains | 45 |
| Total formula weight | 1977859.37 |
| Authors | Fernandez, I.S.,Bai, X.,Scheres, S.H.W.,Ramakrishnan, V. (deposition date: 2014-03-21, release date: 2014-07-09, Last modification date: 2024-11-20) |
| Primary citation | Fernandez, I.S.,Bai, X.,Murshudov, G.,Scheres, S.H.W.,Ramakrishnan, V. Initiation of Translation by Cricket Paralysis Virus Ires Requires its Translocation in the Ribosome. Cell(Cambridge,Mass.), 157:823-, 2014 Cited by PubMed Abstract: The cricket paralysis virus internal ribosome entry site (CrPV-IRES) is a folded structure in a viral mRNA that allows initiation of translation in the absence of any host initiation factors. By using recent advances in single-particle electron cryomicroscopy, we have solved the structure of CrPV-IRES bound to the ribosome of the yeast Kluyveromyces lactis in both the canonical and rotated states at overall resolutions of 3.7 and 3.8 Å, respectively. In both states, the pseudoknot PKI of the CrPV-IRES mimics a tRNA/mRNA interaction in the decoding center of the A site of the 40S ribosomal subunit. The structure and accompanying factor-binding data show that CrPV-IRES binding mimics a pretranslocation rather than initiation state of the ribosome. Translocation of the IRES by elongation factor 2 (eEF2) is required to bring the first codon of the mRNA into the A site and to allow the start of translation. PubMed: 24792965DOI: 10.1016/J.CELL.2014.04.015 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.7 Å) |
Structure validation
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