4V8Y

Cryo-EM reconstruction of the 80S-eIF5B-Met-itRNAMet Eukaryotic Translation Initiation Complex

This is a large structure.

Summary for 4V8Y

Related4BYT 4BYU 4BYV 4BYW 4BYX
Integrates4BYL 4BYN 4BYO 4BYP 4BYQ 4BYR 4BYS
EMDB information2421
Descriptor40S RIBOSOMAL PROTEIN S26-A, 40S RIBOSOMAL PROTEIN S27-A, 40S RIBOSOMAL PROTEIN S28-A, ... (90 entities in total)
Functional Keywordsribosome, ribosome initiation complex, initiator factor eif5b, single particle analysis
Biological sourceSACCHAROMYCES CEREVISIAE (BAKER'S YEAST)
Cellular locationCytoplasm  P32905 P33442 P25443 P05750 P0CX35 P26783 P0CX37 P26786 P0CX39 O13516 Q08745 P0CX47 P48589 P05756 P06367 Q01855 P0CX51 P02407 P0CX55 P07280 P38701 P0C0V8 P0C0W1 P0CX29 P0CX31 Q3E792 P39938 P35997 Q3E7X9 P41057 P0CX33 P38011 P39015 P0CX46 P14126 P10664 P26321 Q02326 P05737 P17076 P05738 P41805 P0C0W9 Q12690 P38754 P05748 P26784 P05740 P0CX49 P0CX83 P0CX24 Q02753 P05749 P0CX41 P04449 P04456 P05743 P0C2H6 P02406 P05747 P14120 P0C2H8 P38061 P05744 P87262 P0CX85 P05745 P49166 P49167 P04650 P0CX87 P0CX27 P05317 P39730
Ubiquitin: Cytoplasm . 40S ribosomal protein S31: Cytoplasm  P05759
Ubiquitin: Cytoplasm . 60S ribosomal protein L40-A: Cytoplasm  P0CH08
Total number of polymer chains87
Total molecular weight3376455.41
Authors
Fernandez, I.S.,Bai, X.C.,Hussain, T.,Kelley, A.C.,Lorsch, J.R.,Ramakrishnan, V.,Scheres, S.H.W. (deposition date: 2013-07-20, release date: 2014-07-09, Last modification date: 2018-04-18)
Primary citation
Fernandez, I.S.,Bai, X.C.,Hussain, T.,Kelley, A.C.,Lorsch, J.R.,Ramakrishnan, V.,Scheres, S.H.W.
Molecular architecture of a eukaryotic translational initiation complex.
Science, 342:1240585-1240585, 2013
PubMed: 24200810 (PDB entries with the same primary citation)
DOI: 10.1126/science.1240585
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY (4.3 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone356.5%21.0%0.43MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 4v8y
no rotation
Molmil generated image of 4v8y
rotated about x axis by 90°
Molmil generated image of 4v8y
rotated about y axis by 90°
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