4V8Q
Complex of SmpB, a tmRNA fragment and EF-Tu-GDP-Kirromycin with the 70S ribosome
This is a non-PDB format compatible entry.
Summary for 4V8Q
| Entry DOI | 10.2210/pdb4v8q/pdb |
| Related | 1GD8 1GIY 1V8Q 1WKI 1YL3 2B66 2B9N 2B9P 2J01 2J03 2V47 2V49 2WDI 2WDJ 2WDL 2WDN 2WH2 2WH4 2WRO 2WRR 2X9S 2X9U 2XG0 2XG2 2XQE 2XTG 2XUX 2Y0V 2Y0X 2Y0Z 2Y11 2Y13 2Y15 2Y17 2Y19 3ZVP |
| Descriptor | 50S RIBOSOMAL PROTEIN L27, 50S RIBOSOMAL PROTEIN L36, 23S ribosomal RNA, ... (63 entities in total) |
| Functional Keywords | tmrna, ribosome, smpb, ef-tu |
| Biological source | Thermus thermophilus HB8 More |
| Total number of polymer chains | 60 |
| Total formula weight | 2348299.52 |
| Authors | Neubauer, C.,Gillet, R.,Kelley, A.C.,Ramakrishnan, V. (deposition date: 2011-12-10, release date: 2014-07-09, Last modification date: 2024-01-10) |
| Primary citation | Neubauer, C.,Gillet, R.,Kelley, A.C.,Ramakrishnan, V. Decoding in the absence of a codon by tmRNA and SmpB in the ribosome. Science, 335:1366-1369, 2012 Cited by PubMed Abstract: In bacteria, ribosomes stalled at the end of truncated messages are rescued by transfer-messenger RNA (tmRNA), a bifunctional molecule that acts as both a transfer RNA (tRNA) and a messenger RNA (mRNA), and SmpB, a small protein that works in concert with tmRNA. Here, we present the crystal structure of a tmRNA fragment, SmpB and elongation factor Tu bound to the ribosome at 3.2 angstroms resolution. The structure shows how SmpB plays the role of both the anticodon loop of tRNA and portions of mRNA to facilitate decoding in the absence of an mRNA codon in the A site of the ribosome and explains why the tmRNA-SmpB system does not interfere with normal translation. PubMed: 22422985DOI: 10.1126/science.1217039 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.1 Å) |
Structure validation
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