4V8Q
Complex of SmpB, a tmRNA fragment and EF-Tu-GDP-Kirromycin with the 70S ribosome
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-27 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 202.170, 290.760, 250.650 |
| Unit cell angles | 90.00, 97.63, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2Y11 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.410 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.240 | 0.700 |
| Number of reflections | 503945 | |
| <I/σ(I)> | 8.8 | 1.48 |
| Completeness [%] | 97.9 | 92.6 |
| Redundancy | 8.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 100 MM MES PH 6.5, 20 MM KCL, 8% (W/V) PEG20K |
