4V8P

T.thermophila 60S ribosomal subunit in complex with initiation factor 6.

これはPDB形式変換不可エントリーです。

4V8P の概要

• エントリーDOI: 10.2210/pdb4v8p/pdb
• 分子名称: 26S RRNA, UBIQUITIN-60S RIBOSOMAL PROTEIN L40, RPL34, ... (50 entities in total)
• 機能のキーワード: ribosome, eukaryotic initiation factor 6, eif6, 60s, translation, large ribosomal subunit, rrna, ribosomal protein
• 由来する生物種: TETRAHYMENA THERMOPHILA; 詳細
• 細胞内の位置: Ubiquitin: Cytoplasm . 60S ribosomal protein L40: Cytoplasm : P0DJ25; Cytoplasm : P0DJ19 P0DJ21 P0DJ18 P0DJ24 P0DJ52 O96774 P0DJ55 Q231U7 P0DJ17 Q22X38 Q245F2
• タンパク質・核酸の鎖数: 184
• 化学式量合計: 7975794.74

構造登録者

Klinge, S.,Voigts-Hoffmann, F.,Leibundgut, M.,Arpagaus, S.,Ban, N. (登録日: 2011-09-14, 公開日: 2014-07-09, 最終更新日: 2024-01-10)

主引用文献

Klinge, S.,Voigts-Hoffmann, F.,Leibundgut, M.,Arpagaus, S.,Ban, N.
Crystal Structure of the Eukaryotic 60S Ribosomal Subunit in Complex with Initiation Factor 6.
Science, 334:941-, 2011

PubMed Abstract: Protein synthesis in all organisms is catalyzed by ribosomes. In comparison to their prokaryotic counterparts, eukaryotic ribosomes are considerably larger and are subject to more complex regulation. The large ribosomal subunit (60S) catalyzes peptide bond formation and contains the nascent polypeptide exit tunnel. We present the structure of the 60S ribosomal subunit from Tetrahymena thermophila in complex with eukaryotic initiation factor 6 (eIF6), cocrystallized with the antibiotic cycloheximide (a eukaryotic-specific inhibitor of protein synthesis), at a resolution of 3.5 angstroms. The structure illustrates the complex functional architecture of the eukaryotic 60S subunit, which comprises an intricate network of interactions between eukaryotic-specific ribosomal protein features and RNA expansion segments. It reveals the roles of eukaryotic ribosomal protein elements in the stabilization of the active site and the extent of eukaryotic-specific differences in other functional regions of the subunit. Furthermore, it elucidates the molecular basis of the interaction with eIF6 and provides a structural framework for further studies of ribosome-associated diseases and the role of the 60S subunit in the initiation of protein synthesis.

PubMed: 22052974
DOI: 10.1126/SCIENCE.1211204

実験手法

X-RAY DIFFRACTION (3.52 Å)