4V8N

The crystal structure of agmatidine tRNA-Ile2 bound to the 70S ribosome in the A and P site.

This is a non-PDB format compatible entry.

Summary for 4V8N

• Entry DOI: 10.2210/pdb4v8n/pdb
• Descriptor: 16S RRNA, 30S RIBOSOMAL PROTEIN S10, 30S RIBOSOMAL PROTEIN S11, ... (58 entities in total)
• Functional Keywords: ribosome, agmatidine, trna, trnaile2
• Biological source: THERMUS THERMOPHILUS; More
• Total number of polymer chains: 116
• Total formula weight: 4584464.10

Authors

Voorhees, R.M.,Mandal, D.,Neubauer, C.,Koehrer, C.,RajBhandary, U.L.,Ramakrishnan, V. (deposition date: 2013-02-13, release date: 2014-07-09, Last modification date: 2024-11-13)

Primary citation

Voorhees, R.M.,Mandal, D.,Neubauer, C.,Koehrer, C.,Rajbhandary, U.L.,Ramakrishnan, V.
The Structural Basis for Specific Decoding of Aua by Isoleucine tRNA on the Ribosome
Nat.Struct.Mol.Biol., 20:641-, 2013

PubMed Abstract: Decoding of the AUA isoleucine codon in bacteria and archaea requires modification of a C in the anticodon wobble position of the isoleucine tRNA. Here, we report the crystal structure of the archaeal tRNA2(Ile), which contains the modification agmatidine in its anticodon, in complex with the AUA codon on the 70S ribosome. The structure illustrates how agmatidine confers codon specificity for AUA over AUG.

PubMed: 23542153
DOI: 10.1038/NSMB.2545

Experimental method

X-RAY DIFFRACTION (3.1 Å)