4V8N
The crystal structure of agmatidine tRNA-Ile2 bound to the 70S ribosome in the A and P site.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-02 |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 211.900, 450.790, 625.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| R-factor | 0.25 |
| Rwork | 0.250 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2J00 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.020 | 1.200 |
| Number of reflections | 1046021 | |
| <I/σ(I)> | 8.5 | 1.1 |
| Completeness [%] | 97.7 | 76.1 |
| Redundancy | 5.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.1 | 0.1 M TRIS-ACETATE PH 7, 0.2 M KSCN, 3.5-5.5% (W/V) PEG 20K, AND 3.5-5.5% (W/V) PEG 550 MONOMETHYL ETHER |
