4V84

Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome.

これはPDB形式変換不可エントリーです。

4V84 の概要

• エントリーDOI: 10.2210/pdb4v84/pdb
• 関連するPDBエントリー: 3PYN 3PYO 3PYQ 3PYR 3PYT 3PYU 3PYV
• 分子名称: ribosomal RNA 16S, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (55 entities in total)
• 機能のキーワード: 70s, ribosome, igr, ires, psiv, crpv
• 由来する生物種: Thermus thermophilus HB27; 詳細
• タンパク質・核酸の鎖数: 106
• 化学式量合計: 4282688.60

構造登録者

Zhu, J.,Korostelev, A.,Costantino, D.,Noller, H.F.,Kieft, J.S. (登録日: 2010-12-13, 公開日: 2014-07-09, 最終更新日: 2024-10-30)

主引用文献

Zhu, J.,Korostelev, A.,Costantino, D.A.,Donohue, J.P.,Noller, H.F.,Kieft, J.S.
Crystal structures of complexes containing domains from two viral internal ribosome entry site (IRES) RNAs bound to the 70S ribosome.
Proc.Natl.Acad.Sci.USA, 108:1839-1844, 2011

PubMed Abstract: Internal ribosome entry site (IRES) RNAs are elements of viral or cellular mRNAs that bypass steps of canonical eukaryotic cap-dependent translation initiation. Understanding of the structural basis of IRES mechanisms is limited, partially due to a lack of high-resolution structures of IRES RNAs bound to their cellular targets. Prompted by the universal phylogenetic conservation of the ribosomal P site, we solved the crystal structures of proposed P site binding domains from two intergenic region IRES RNAs bound to bacterial 70S ribosomes. The structures show that these IRES domains nearly perfectly mimic a tRNA • mRNA interaction. However, there are clear differences in the global shape and position of this IRES domain in the intersubunit space compared to those of tRNA, supporting a mechanism for IRES action that invokes hybrid state mimicry to drive a noncanonical mode of translocation. These structures suggest how relatively small structured RNAs can manipulate complex biological machines.

PubMed: 21245352
DOI: 10.1073/pnas.1018582108

実験手法

X-RAY DIFFRACTION (3.4 Å)