4V7H

Structure of the 80S rRNA and proteins and P/E tRNA for eukaryotic ribosome based on cryo-EM map of Thermomyces lanuginosus ribosome at 8.9A resolution

これはPDB形式変換不可エントリーです。

4V7H の概要

• エントリーDOI: 10.2210/pdb4v7h/pdb
• EMDBエントリー: 1345
• 分子名称: 18S rRNA, 40S ribosomal protein S14(A), 40S ribosomal protein S23(A), ... (53 entities in total)
• 機能のキーワード: eukaryotic ribosome, 80s, rack1 protein, flexible fitting, ribosome
• 由来する生物種: Thermomyces lanuginosus; 詳細
• タンパク質・核酸の鎖数: 53
• 化学式量合計: 2462331.63

構造登録者

Taylor, D.J.,Devkota, B.,Huang, A.D.,Topf, M.,Narayanan, E.,Sali, A.,Harvey, S.C.,Frank, J. (登録日: 2009-09-22, 公開日: 2014-07-09, 最終更新日: 2024-02-28)

主引用文献

Taylor, D.J.,Devkota, B.,Huang, A.D.,Topf, M.,Narayanan, E.,Sali, A.,Harvey, S.C.,Frank, J.
Comprehensive molecular structure of the eukaryotic ribosome.
Structure, 17:1591-1604, 2009

PubMed Abstract: Despite the emergence of a large number of X-ray crystallographic models of the bacterial 70S ribosome over the past decade, an accurate atomic model of the eukaryotic 80S ribosome is still not available. Eukaryotic ribosomes possess more ribosomal proteins and ribosomal RNA than do bacterial ribosomes, which are implicated in extraribosomal functions in the eukaryotic cells. By combining cryo-EM with RNA and protein homology modeling, we obtained an atomic model of the yeast 80S ribosome complete with all ribosomal RNA expansion segments and all ribosomal proteins for which a structural homolog can be identified. Mutation or deletion of 80S ribosomal proteins can abrogate maturation of the ribosome, leading to several human diseases. We have localized one such protein unique to eukaryotes, rpS19e, whose mutations are associated with Diamond-Blackfan anemia in humans. Additionally, we characterize crucial interactions between the dynamic stalk base of the ribosome with eukaryotic elongation factor 2.

PubMed: 20004163
DOI: 10.1016/j.str.2009.09.015

実験手法

ELECTRON MICROSCOPY (8.9 Å)