4V6I
Localization of the small subunit ribosomal proteins into a 6.1 A cryo-EM map of Saccharomyces cerevisiae translating 80S ribosome
This is a non-PDB format compatible entry.
Replaces: 3IZCSummary for 4V6I
Entry DOI | 10.2210/pdb4v6i/pdb |
Related | 3IZ5 3IZ6 3IZ7 3IZ9 3IZD |
EMDB information | 1667 1669 1811 |
Descriptor | 40S ribosomal protein RACK1 (RACK1), 40S ribosomal protein rpS16 (S9p), 40S ribosomal protein rpS20 (S10p), ... (83 entities in total) |
Functional Keywords | eukaryotic ribosome, homology modeling, de novo modeling, ribosomal proteins, novel ribosomal proteins, ribosome |
Biological source | Saccharomyces cerevisiae (yeast) More |
Total number of polymer chains | 86 |
Total formula weight | 3104129.58 |
Authors | Armache, J.-P.,Jarasch, A.,Anger, A.M.,Villa, E.,Becker, T.,Bhushan, S.,Jossinet, F.,Habeck, M.,Dindar, G.,Franckenberg, S.,Marquez, V.,Mielke, T.,Thomm, M.,Berninghausen, O.,Beatrix, B.,Soeding, J.,Westhof, E.,Wilson, D.N.,Beckmann, R. (deposition date: 2010-10-12, release date: 2014-07-09, Last modification date: 2024-02-28) |
Primary citation | Armache, J.P.,Jarasch, A.,Anger, A.M.,Villa, E.,Becker, T.,Bhushan, S.,Jossinet, F.,Habeck, M.,Dindar, G.,Franckenberg, S.,Marquez, V.,Mielke, T.,Thomm, M.,Berninghausen, O.,Beatrix, B.,Soding, J.,Westhof, E.,Wilson, D.N.,Beckmann, R. Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution. Proc.Natl.Acad.Sci.USA, 107:19748-19753, 2010 Cited by PubMed Abstract: Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution. This map, together with a 6.1-Å map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ∼98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome. PubMed: 20980660DOI: 10.1073/pnas.1009999107 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (8.8 Å) |
Structure validation
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