4V5Z
Structure of a mammalian 80S ribosome obtained by docking homology models of the RNA and proteins into an 8.7 A cryo-EM map
This is a non-PDB format compatible entry.
Summary for 4V5Z
Entry DOI | 10.2210/pdb4v5z/pdb |
Related | 2ZKR |
EMDB information | 1480 |
Descriptor | 18S Ribosomal RNA, 40S Ribosomal protein SA, 40S Ribosomal protein S3e, ... (86 entities in total) |
Functional Keywords | protein-rna complex, 40s ribosomal subunit, ribosomal protein-rna complex, ribosomal protein/rna |
Biological source | Canis familiaris (dog) More |
Total number of polymer chains | 86 |
Total formula weight | 2902274.52 |
Authors | Chandramouli, P.,Akey, C.W. (deposition date: 2008-03-27, release date: 2014-07-09, Last modification date: 2024-03-20) |
Primary citation | Chandramouli, P.,Topf, M.,Menetret, J.F.,Eswar, N.,Cannone, J.J.,Gutell, R.R.,Sali, A.,Akey, C.W. Structure of the Mammalian 80S Ribosome at 8.7 A Resolution Structure, 16:535-548, 2008 Cited by PubMed Abstract: In this paper, we present a structure of the mammalian ribosome determined at approximately 8.7 A resolution by electron cryomicroscopy and single-particle methods. A model of the ribosome was created by docking homology models of subunit rRNAs and conserved proteins into the density map. We then modeled expansion segments in the subunit rRNAs and found unclaimed density for approximately 20 proteins. In general, many conserved proteins and novel proteins interact with expansion segments to form an integrated framework that may stabilize the mature ribosome. Our structure provides a snapshot of the mammalian ribosome at the beginning of translation and lends support to current models in which large movements of the small subunit and L1 stalk occur during tRNA translocation. Finally, details are presented for intersubunit bridges that are specific to the eukaryotic ribosome. We suggest that these bridges may help reset the conformation of the ribosome to prepare for the next cycle of chain elongation. PubMed: 18400176DOI: 10.1016/j.str.2008.01.007 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (8.7 Å) |
Structure validation
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