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4V5Z

Structure of a mammalian 80S ribosome obtained by docking homology models of the RNA and proteins into an 8.7 A cryo-EM map

This is a non-PDB format compatible entry.
Summary for 4V5Z
Entry DOI10.2210/pdb4v5z/pdb
Related2ZKR
EMDB information1480
Descriptor18S Ribosomal RNA, 40S Ribosomal protein SA, 40S Ribosomal protein S3e, ... (86 entities in total)
Functional Keywordsprotein-rna complex, 40s ribosomal subunit, ribosomal protein-rna complex, ribosomal protein/rna
Biological sourceCanis familiaris (dog)
More
Total number of polymer chains86
Total formula weight2902274.52
Authors
Chandramouli, P.,Akey, C.W. (deposition date: 2008-03-27, release date: 2014-07-09, Last modification date: 2024-03-20)
Primary citationChandramouli, P.,Topf, M.,Menetret, J.F.,Eswar, N.,Cannone, J.J.,Gutell, R.R.,Sali, A.,Akey, C.W.
Structure of the Mammalian 80S Ribosome at 8.7 A Resolution
Structure, 16:535-548, 2008
Cited by
PubMed Abstract: In this paper, we present a structure of the mammalian ribosome determined at approximately 8.7 A resolution by electron cryomicroscopy and single-particle methods. A model of the ribosome was created by docking homology models of subunit rRNAs and conserved proteins into the density map. We then modeled expansion segments in the subunit rRNAs and found unclaimed density for approximately 20 proteins. In general, many conserved proteins and novel proteins interact with expansion segments to form an integrated framework that may stabilize the mature ribosome. Our structure provides a snapshot of the mammalian ribosome at the beginning of translation and lends support to current models in which large movements of the small subunit and L1 stalk occur during tRNA translocation. Finally, details are presented for intersubunit bridges that are specific to the eukaryotic ribosome. We suggest that these bridges may help reset the conformation of the ribosome to prepare for the next cycle of chain elongation.
PubMed: 18400176
DOI: 10.1016/j.str.2008.01.007
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (8.7 Å)
Structure validation

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數據於2025-07-09公開中

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