4V5X

The cryo-EM structure of a 3D DNA-origami object

これはPDB形式変換不可エントリーです。

4V5X の概要

• エントリーDOI: 10.2210/pdb4v5x/pdb
• EMDBエントリー: 2210
• 分子名称: SCAFFOLD STRAND,SCAFFOLD STRAND, STAPLE STRAND, ... (162 entities in total)
• 機能のキーワード: dna, dna-templated design, synthetic biology
• 由来する生物種: ENTEROBACTERIA PHAGE M13 (M13 PHAGE); 詳細
• タンパク質・核酸の鎖数: 162
• 化学式量合計: 4692024.27

構造登録者

Bai, X.C.,Martin, T.G.,Scheres, S.H.W.,Dietz, H. (登録日: 2012-10-09, 公開日: 2014-07-09, 最終更新日: 2024-05-08)

主引用文献

Bai, X.,Martin, T.G.,Scheres, S.H.W.,Dietz, H.
Cryo-Em Structure of a 3D DNA-Origami Object.
Proc.Natl.Acad.Sci.USA, 109:20012-, 2012

PubMed Abstract: A key goal for nanotechnology is to design synthetic objects that may ultimately achieve functionalities known today only from natural macromolecular complexes. Molecular self-assembly with DNA has shown potential for creating user-defined 3D scaffolds, but the level of attainable positional accuracy has been unclear. Here we report the cryo-EM structure and a full pseudoatomic model of a discrete DNA object that is almost twice the size of a prokaryotic ribosome. The structure provides a variety of stable, previously undescribed DNA topologies for future use in nanotechnology and experimental evidence that discrete 3D DNA scaffolds allow the positioning of user-defined structural motifs with an accuracy that is similar to that observed in natural macromolecules. Thereby, our results indicate an attractive route to fabricate nanoscale devices that achieve complex functionalities by DNA-templated design steered by structural feedback.

PubMed: 23169645
DOI: 10.1073/PNAS.1215713109

実験手法

ELECTRON MICROSCOPY (11.5 Å)