4V5H
E.Coli 70s Ribosome Stalled During Translation Of Tnac Leader Peptide.
This is a non-PDB format compatible entry.
Summary for 4V5H
| Entry DOI | 10.2210/pdb4v5h/pdb |
| Related | 1M5G 1MJ1 1P6G 1P87 1S03 1ZN1 2AVY 2AW7 2VHO 2VHP |
| EMDB information | 1657 |
| Descriptor | 16S RIBOSOMAL RNA, 30S RIBOSOMAL PROTEIN S10, 30S RIBOSOMAL PROTEIN S11, ... (56 entities in total) |
| Functional Keywords | ribosomal protein, ribonucleoprotein, nucleotide-binding, protein biosynthesis, translation, zinc-finger, tnac, 70s ribosome, stalling, ribosome |
| Biological source | ESCHERICHIA COLI More |
| Total number of polymer chains | 56 |
| Total formula weight | 2166081.17 |
| Authors | Seidelt, B.,Innis, C.A.,Wilson, D.N.,Gartmann, M.,Armache, J.,Villa, E.,Trabuco, L.G.,Becker, T.,Mielke, T.,Schulten, K.,Steitz, T.A.,Beckmann, R. (deposition date: 2009-10-26, release date: 2014-07-09, Last modification date: 2024-05-08) |
| Primary citation | Seidelt, B.,Innis, C.A.,Wilson, D.N.,Gartmann, M.,Armache, J.P.,Villa, E.,Trabuco, L.G.,Becker, T.,Mielke, T.,Schulten, K.,Steitz, T.A.,Beckmann, R. Structural insight into nascent polypeptide chain-mediated translational stalling. Science, 326:1412-1415, 2009 Cited by PubMed Abstract: Expression of the Escherichia coli tryptophanase operon depends on ribosome stalling during translation of the upstream TnaC leader peptide, a process for which interactions between the TnaC nascent chain and the ribosomal exit tunnel are critical. We determined a 5.8 angstrom-resolution cryo-electron microscopy and single-particle reconstruction of a ribosome stalled during translation of the tnaC leader gene. The nascent chain was extended within the exit tunnel, making contacts with ribosomal components at distinct sites. Upon stalling, two conserved residues within the peptidyltransferase center adopted conformations that preclude binding of release factors. We propose a model whereby interactions within the tunnel are relayed to the peptidyltransferase center to inhibit translation. Moreover, we show that nascent chains adopt distinct conformations within the ribosomal exit tunnel. PubMed: 19933110DOI: 10.1126/science.1177662 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (5.8 Å) |
Structure validation
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