4V5H

E.Coli 70s Ribosome Stalled During Translation Of Tnac Leader Peptide.

これはPDB形式変換不可エントリーです。

4V5H の概要

• エントリーDOI: 10.2210/pdb4v5h/pdb
• 関連するPDBエントリー: 1M5G 1MJ1 1P6G 1P87 1S03 1ZN1 2AVY 2AW7 2VHO 2VHP
• EMDBエントリー: 1657
• 分子名称: 16S RIBOSOMAL RNA, 30S RIBOSOMAL PROTEIN S10, 30S RIBOSOMAL PROTEIN S11, ... (56 entities in total)
• 機能のキーワード: ribosomal protein, ribonucleoprotein, nucleotide-binding, protein biosynthesis, translation, zinc-finger, tnac, 70s ribosome, stalling, ribosome
• 由来する生物種: ESCHERICHIA COLI; 詳細
• タンパク質・核酸の鎖数: 56
• 化学式量合計: 2166081.17

構造登録者

Seidelt, B.,Innis, C.A.,Wilson, D.N.,Gartmann, M.,Armache, J.,Villa, E.,Trabuco, L.G.,Becker, T.,Mielke, T.,Schulten, K.,Steitz, T.A.,Beckmann, R. (登録日: 2009-10-26, 公開日: 2014-07-09, 最終更新日: 2024-05-08)

主引用文献

Seidelt, B.,Innis, C.A.,Wilson, D.N.,Gartmann, M.,Armache, J.P.,Villa, E.,Trabuco, L.G.,Becker, T.,Mielke, T.,Schulten, K.,Steitz, T.A.,Beckmann, R.
Structural insight into nascent polypeptide chain-mediated translational stalling.
Science, 326:1412-1415, 2009

PubMed Abstract: Expression of the Escherichia coli tryptophanase operon depends on ribosome stalling during translation of the upstream TnaC leader peptide, a process for which interactions between the TnaC nascent chain and the ribosomal exit tunnel are critical. We determined a 5.8 angstrom-resolution cryo-electron microscopy and single-particle reconstruction of a ribosome stalled during translation of the tnaC leader gene. The nascent chain was extended within the exit tunnel, making contacts with ribosomal components at distinct sites. Upon stalling, two conserved residues within the peptidyltransferase center adopted conformations that preclude binding of release factors. We propose a model whereby interactions within the tunnel are relayed to the peptidyltransferase center to inhibit translation. Moreover, we show that nascent chains adopt distinct conformations within the ribosomal exit tunnel.

PubMed: 19933110
DOI: 10.1126/science.1177662

実験手法

ELECTRON MICROSCOPY (5.8 Å)