4V57

Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin and neomycin.

これはPDB形式変換不可エントリーです。

4V57 の概要

• エントリーDOI: 10.2210/pdb4v57/pdb
• 関連するPDBエントリー: 2QOZ 2QP0 2QP1
• 分子名称: 16S rRNA, 30S ribosomal protein S11, 30S ribosomal protein S12, ... (57 entities in total)
• 機能のキーワード: rna-protein complex, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 104
• 化学式量合計: 4298300.88

構造登録者

Borovinskaya, M.A.,Shoji, S.,Holton, J.M.,Fredrick, K.,Cate, J.H.D. (登録日: 2007-07-21, 公開日: 2014-07-09, 最終更新日: 2023-09-20)

主引用文献

Borovinskaya, M.A.,Shoji, S.,Holton, J.M.,Fredrick, K.,Cate, J.H.
A steric block in translation caused by the antibiotic spectinomycin.
Acs Chem.Biol., 2:545-552, 2007

PubMed Abstract: The widely used antibiotic spectinomycin inhibits bacterial protein synthesis by blocking translocation of messenger RNA and transfer RNAs on the ribosome. Here, we show that in crystals of the Escherichia coli 70S ribosome spectinomycin binding traps a distinct swiveling state of the head domain of the small ribosomal subunit. Spectinomycin also alters the rate and completeness of reverse translocation in vitro. These structural and biochemical data indicate that in solution spectinomycin sterically blocks swiveling of the head domain of the small ribosomal subunit and thereby disrupts the translocation cycle.

PubMed: 17696316
DOI: 10.1021/cb700100n

実験手法

X-RAY DIFFRACTION (3.5 Å)