4V4P

Crystal structure of 70S ribosome with thrS operator and tRNAs.

これはPDB形式変換不可エントリーです。

4V4P の概要

• エントリーDOI: 10.2210/pdb4v4p/pdb
• 関連するPDBエントリー: 1YL4 1giy 1nkw
• 分子名称: 5S ribosomal RNA, 50S ribosomal protein L9, 50S ribosomal protein L11, ... (56 entities in total)
• 機能のキーワード: ribosome, mrna, translational operator
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 58
• 化学式量合計: 2308735.78

構造登録者

Jenner, L.,Romby, P.,Rees, B.,Schulze-Briese, C.,Springer, M.,Ehresmann, C.,Ehresmann, B.,Moras, D.,Yusupova, G.,Yusupov, M. (登録日: 2005-01-19, 公開日: 2014-07-09, 最終更新日: 2024-10-30)

主引用文献

Jenner, L.,Romby, P.,Rees, B.,Schulze-Briese, C.,Springer, M.,Ehresmann, C.,Ehresmann, B.,Moras, D.,Yusupova, G.,Yusupov, M.
Translational operator of mRNA on the ribosome: how repressor proteins exclude ribosome binding.
Science, 308:120-123, 2005

PubMed Abstract: The ribosome of Thermus thermophilus was cocrystallized with initiator transfer RNA (tRNA) and a structured messenger RNA (mRNA) carrying a translational operator. The path of the mRNA was defined at 5.5 angstroms resolution by comparing it with either the crystal structure of the same ribosomal complex lacking mRNA or with an unstructured mRNA. A precise ribosomal environment positions the operator stem-loop structure perpendicular to the surface of the ribosome on the platform of the 30S subunit. The binding of the operator and of the initiator tRNA occurs on the ribosome with an unoccupied tRNA exit site, which is expected for an initiation complex. The positioning of the regulatory domain of the operator relative to the ribosome elucidates the molecular mechanism by which the bound repressor switches off translation. Our data suggest a general way in which mRNA control elements must be placed on the ribosome to perform their regulatory task.

PubMed: 15802605
DOI: 10.1126/science.1105639

実験手法

X-RAY DIFFRACTION (5.5 Å)