4V4L

Structure of the Drosophila apoptosome

これはPDB形式変換不可エントリーです。

4V4L の概要

• エントリーDOI: 10.2210/pdb4v4l/pdb
• EMDBエントリー: 5235
• 分子名称: Apaf-1 related killer DARK, MAGNESIUM ION, 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE (3 entities in total)
• 機能のキーワード: drosophila apoptosome, apoptosis, programmed cell death
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 1979376.29

構造登録者

Yuan, S.,Topf, M.,Akey, C.W.,Ludtke, S.J. (登録日: 2010-10-04, 公開日: 2014-07-09, 最終更新日: 2024-02-28)

主引用文献

Yuan, S.,Yu, X.,Topf, M.,Dorstyn, L.,Kumar, S.,Ludtke, S.J.,Akey, C.W.
Structure of the Drosophila apoptosome at 6.9 angstrom resolution
Structure, 19:128-140, 2011

PubMed Abstract: The Drosophila Apaf-1 related killer forms an apoptosome in the intrinsic cell death pathway. In this study we show that Dark forms a single ring when initiator procaspases are bound. This Dark-Dronc complex cleaves DrICE efficiently; hence, a single ring represents the Drosophila apoptosome. We then determined the 3D structure of a double ring at ∼6.9 Å resolution and created a model of the apoptosome. Subunit interactions in the Dark complex are similar to those in Apaf-1 and CED-4 apoptosomes, but there are significant differences. In particular, Dark has "lost" a loop in the nucleotide-binding pocket, which opens a path for possible dATP exchange in the apoptosome. In addition, caspase recruitment domains (CARDs) form a crown on the central hub of the Dark apoptosome. This CARD geometry suggests that conformational changes will be required to form active Dark-Dronc complexes. When taken together, these data provide insights into apoptosome structure, function, and evolution.

PubMed: 21220123
DOI: 10.1016/j.str.2010.10.009

実験手法

ELECTRON MICROSCOPY (6.9 Å)