4V4J

Interactions and Dynamics of the Shine-Dalgarno Helix in the 70S Ribosome.

これはPDB形式変換不可エントリーです。

4V4J の概要

• エントリーDOI: 10.2210/pdb4v4j/pdb
• 関連するPDBエントリー: 2QNH
• 分子名称: 23S LARGE SUBUNIT RIBOSOMAL RNA, 50S ribosomal protein L13, 50S ribosomal protein L14, ... (54 entities in total)
• 機能のキーワード: 70s ribosome, shine-dalgarno, ribosome
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 54
• 化学式量合計: 2224546.50

構造登録者

Korostelev, A.,Trakhanov, S.,Asahara, H.,Laurberg, M.,Noller, H.F. (登録日: 2007-07-18, 公開日: 2014-07-09, 最終更新日: 2024-10-09)

主引用文献

Korostelev, A.,Trakhanov, S.,Asahara, H.,Laurberg, M.,Lancaster, L.,Noller, H.F.
Interactions and dynamics of the Shine Dalgarno helix in the 70S ribosome.
Proc.Natl.Acad.Sci.Usa, 104:16840-16843, 2007

PubMed Abstract: The crystal structure of an initiation-like 70S ribosome complex containing an 8-bp Shine-Dalgarno (SD) helix was determined at 3.8-A resolution. Translation-libration-screw analysis showed that the inherent anisotropic motions of the SD helix were biased along its helical axis, suggesting that during the first step of translocation, the SD helix moves along its helical screw axis. Contacts between the SD helix and the ribosome were primarily through interactions with helices 23a, 26, and 28 of 16S rRNA. Contact with the neck (helix 28) of the 30S subunit near its hinge point suggests that formation of the SD helix could affect positioning of the head of the 30S subunit for optimal interaction with initiator tRNA. The bulged U723 in helix 23a interacts with the minor groove of the SD helix at the C1539.G-10 base pair, explaining its selective conservation in bacteria and archaea.

PubMed: 17940016
DOI: 10.1073/pnas.0707850104

実験手法

X-RAY DIFFRACTION (3.83 Å)