4V4H

Crystal structure of the bacterial ribosome from Escherichia coli in complex with the antibiotic kasugamyin at 3.5A resolution.

これはPDB形式変換不可エントリーです。

4V4H の概要

• エントリーDOI: 10.2210/pdb4v4h/pdb
• 関連するPDBエントリー: 1VS6 1VS7 1VS8
• 分子名称: 16S RIBOSOMAL RNA, 30S RIBOSOMAL PROTEIN S11, 30S RIBOSOMAL PROTEIN S12, ... (55 entities in total)
• 機能のキーワード: ribosome, kasugamycin
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 104
• 化学式量合計: 4301002.66

構造登録者

Schuwirth, B.S.,Vila-Sanjurjo, A.,Cate, J.H.D. (登録日: 2006-08-04, 公開日: 2014-07-09, 最終更新日: 2023-09-20)

主引用文献

Schuwirth, B.S.,Day, J.M.,Hau, C.W.,Janssen, G.R.,Dahlberg, A.E.,Cate, J.H.D.,Vila-Sanjurjo, A.
Structural analysis of kasugamycin inhibition of translation.
Nat.Struct.Mol.Biol., 13:879-886, 2006

PubMed Abstract: The prokaryotic ribosome is an important target of antibiotic action. We determined the X-ray structure of the aminoglycoside kasugamycin (Ksg) in complex with the Escherichia coli 70S ribosome at 3.5-A resolution. The structure reveals that the drug binds within the messenger RNA channel of the 30S subunit between the universally conserved G926 and A794 nucleotides in 16S ribosomal RNA, which are sites of Ksg resistance. To our surprise, Ksg resistance mutations do not inhibit binding of the drug to the ribosome. The present structural and biochemical results indicate that inhibition by Ksg and Ksg resistance are closely linked to the structure of the mRNA at the junction of the peptidyl-tRNA and exit-tRNA sites (P and E sites).

PubMed: 16998486
DOI: 10.1038/nsmb1150

実験手法

X-RAY DIFFRACTION (3.46 Å)