4V42

Crystal structure of the ribosome at 5.5 A resolution.

これはPDB形式変換不可エントリーです。

4V42 の概要

• エントリーDOI: 10.2210/pdb4v42/pdb
• 関連するPDBエントリー: 486D
• 分子名称: 30S 16S RIBOSOMAL RNA, 30S RIBOSOMAL PROTEIN S7, 30S RIBOSOMAL PROTEIN S8, ... (47 entities in total)
• 機能のキーワード: ribosome assembly, protein synthesis, life, ribosome
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 49
• 化学式量合計: 2203915.32

構造登録者

Yusupov, M.M.,Yusupova, G.Z.,Baucom, A.,Lieberman, K.,Earnest, T.N.,Cate, J.H.D.,Noller, H.F. (登録日: 2001-03-30, 公開日: 2014-07-09, 最終更新日: 2024-05-22)

主引用文献

Yusupov, M.M.,Yusupova, G.Z.,Baucom, A.,Lieberman, K.,Earnest, T.N.,Cate, J.H.D.,Noller, H.F.
Crystal structure of the ribosome at 5.5 A resolution
Science, 292:883-896, 2001

PubMed Abstract: We describe the crystal structure of the complete Thermus thermophilus 70S ribosome containing bound messenger RNA and transfer RNAs (tRNAs) at 5.5 angstrom resolution. All of the 16S, 23S, and 5S ribosomal RNA (rRNA) chains, the A-, P-, and E-site tRNAs, and most of the ribosomal proteins can be fitted to the electron density map. The core of the interface between the 30S small subunit and the 50S large subunit, where the tRNA substrates are bound, is dominated by RNA, with proteins located mainly at the periphery, consistent with ribosomal function being based on rRNA. In each of the three tRNA binding sites, the ribosome contacts all of the major elements of tRNA, providing an explanation for the conservation of tRNA structure. The tRNAs are closely juxtaposed with the intersubunit bridges, in a way that suggests coupling of the 20 to 50 angstrom movements associated with tRNA translocation with intersubunit movement.

PubMed: 11283358
DOI: 10.1126/science.1060089

実験手法

X-RAY DIFFRACTION (5.5 Å)