4UXF
Crystal structure of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34, P21 native crystal
Summary for 4UXF
Entry DOI | 10.2210/pdb4uxf/pdb |
Related | 4UXE 4UXG |
Descriptor | LARGE TAIL FIBER PROTEIN P34, GLYCEROL (3 entities in total) |
Functional Keywords | viral protein, caudovirales, myoviridae, triple beta-helix |
Biological source | ENTEROBACTERIA PHAGE T4 |
Cellular location | Virion : P18771 |
Total number of polymer chains | 3 |
Total formula weight | 134324.58 |
Authors | Granell, M.,Alvira, S.,Garcia-Doval, C.,Singh, A.K.,van Raaij, M.J. (deposition date: 2014-08-22, release date: 2015-12-16, Last modification date: 2024-05-08) |
Primary citation | Granell, M.,Namura, M.,Alvira, S.,Kanamaru, S.,van Raaij, M.J. Crystal Structure of the Carboxy-Terminal Region of the Bacteriophage T4 Proximal Long Tail Fiber Protein Gp34. Viruses, 9:-, 2017 Cited by PubMed Abstract: Long tail fibers of bacteriophage T4 are formed by proteins gp34, gp35, gp36, and gp37, with gp34 located at the phage-proximal end and gp37 at the phage-distal, receptor-binding end. We have solved the structure of the carboxy-terminal region of gp34, consisting of amino acids 894-1289, by single-wavelength anomalous diffraction and extended the structure to amino acids 744-1289 using data collected from crystals containing longer gp34-fragments. The structure reveals three repeats of a mixed α-β fibrous domain in residues 744 to 877. A triple-helical neck connects to an extended triple β-helix domain (amino acids 900-1127) punctuated by two β-prism domains. Next, a β-prism domain decorated with short helices and extended β-helices is present (residues 1146-1238), while the -terminal end is capped with another short β-helical region and three β-hairpins. The structure provides insight into the stability of the fibrous gp34 protein. PubMed: 28665339DOI: 10.3390/v9070168 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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