4UWE

Structure of the ryanodine receptor at resolution of 8.5 A in partially open state

4UWE の概要

• エントリーDOI: 10.2210/pdb4uwe/pdb
• 関連するPDBエントリー: 4UWA
• EMDBエントリー: 2752
• 分子名称: RYANODINE RECEPTOR 1 (1 entity in total)
• 機能のキーワード: signaling protein, calcium binding, ion channel, muscular contraction, conformational changes.
• 由来する生物種: ORYCTOLAGUS CUNICULUS (RABBIT)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 1894515.62

構造登録者

Efremov, R.G.,Leitner, A.,Aebersold, R.,Raunser, S. (登録日: 2014-08-11, 公開日: 2014-12-10, 最終更新日: 2024-05-08)

主引用文献

Efremov, R.G.,Leitner, A.,Aebersold, R.,Raunser, S.
Architecture and Conformational Switch Mechanism of the Ryanodine Receptor.
Nature, 517:39-, 2015

PubMed Abstract: Muscle contraction is initiated by the release of calcium (Ca(2+)) from the sarcoplasmic reticulum into the cytoplasm of myocytes through ryanodine receptors (RyRs). RyRs are homotetrameric channels with a molecular mass of more than 2.2 megadaltons that are regulated by several factors, including ions, small molecules and proteins. Numerous mutations in RyRs have been associated with human diseases. The molecular mechanism underlying the complex regulation of RyRs is poorly understood. Using electron cryomicroscopy, here we determine the architecture of rabbit RyR1 at a resolution of 6.1 Å. We show that the cytoplasmic moiety of RyR1 contains two large α-solenoid domains and several smaller domains, with folds suggestive of participation in protein-protein interactions. The transmembrane domain represents a chimaera of voltage-gated sodium and pH-activated ion channels. We identify the calcium-binding EF-hand domain and show that it functions as a conformational switch allosterically gating the channel.

PubMed: 25470059
DOI: 10.1038/NATURE13916

実験手法

ELECTRON MICROSCOPY (8.5 Å)