4UT6

Crystal structure of dengue 2 virus envelope glycoprotein in complex with the Fab fragment of the broadly neutralizing human antibody EDE2 B7

4UT6 の概要

• エントリーDOI: 10.2210/pdb4ut6/pdb
• 関連するPDBエントリー: 4UT7 4UT8 4UT9
• 分子名称: ENVELOPE GLYCOPROTEIN E, BROADLY NEUTRALIZING HUMAN ANTIBODY EDE2 B7, BROADLY NEUTRALIZING HUMAN ANTIBODY EDE2, ... (6 entities in total)
• 機能のキーワード: viral protein, membrane fusion, class 2 fusion protein, dengue antibody neutralization, immune system, immune system-viral protein complex
• 由来する生物種: DENGUE VIRUS 2; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 202422.84

構造登録者

Rouvinski, A.,Guardado-Calvo, P.,Barba-Spaeth, G.,Duquerroy, S.,Vaney, M.C.,Rey, F.A. (登録日: 2014-07-18, 公開日: 2015-01-28, 最終更新日: 2024-10-23)

主引用文献

Rouvinski, A.,Guardado-Calvo, P.,Barba-Spaeth, G.,Duquerroy, S.,Vaney, M.,Kikuti, C.M.,Sanchez, M.E.N.,Dejnirattisai, W.,Wongwiwat, W.,Haouz, A.,Girard-Blanc, C.,Petres, S.,Shepard, W.E.,Despres, P.,Arenzana-Seisdedos, F.,Dussart, P.,Mongkolsapaya, J.,Screaton, G.R.,Rey, F.A.
Recognition Determinants of Broadly Neutralizing Human Antibodies Against Dengue Viruses.
Nature, 520:109-, 2015

PubMed Abstract: Dengue disease is caused by four different flavivirus serotypes, which infect 390 million people yearly with 25% symptomatic cases and for which no licensed vaccine is available. Recent phase III vaccine trials showed partial protection, and in particular no protection for dengue virus serotype 2 (refs 3, 4). Structural studies so far have characterized only epitopes recognized by serotype-specific human antibodies. We recently isolated human antibodies potently neutralizing all four dengue virus serotypes. Here we describe the X-ray structures of four of these broadly neutralizing antibodies in complex with the envelope glycoprotein E from dengue virus serotype 2, revealing that the recognition determinants are at a serotype-invariant site at the E-dimer interface, including the exposed main chain of the E fusion loop and the two conserved glycan chains. This 'E-dimer-dependent epitope' is also the binding site for the viral glycoprotein prM during virus maturation in the secretory pathway of the infected cell, explaining its conservation across serotypes and highlighting an Achilles' heel of the virus with respect to antibody neutralization. These findings will be instrumental for devising novel immunogens to protect simultaneously against all four serotypes of dengue virus.

PubMed: 25581790
DOI: 10.1038/NATURE14130

実験手法

X-RAY DIFFRACTION (3.2 Å)