4UQ2

Crystal structure of HLA-A1101 in complex with an azobenzene- containing peptide

Summary for 4UQ2

• Entry DOI: 10.2210/pdb4uq2/pdb
• Related: 4UQ3
• Descriptor: HLA CLASS I HISTOCOMPATIBILITY ANTIGEN, A-11 ALPHA CHAIN, BETA-2-MICROGLOBULIN, AZOBENZENE-CONTAINING PEPTIDE, ... (4 entities in total)
• Functional Keywords: azobenzene, hla-a, synthetic peptide, immune system
• Biological source: HOMO SAPIENS (HUMAN); More
• Cellular location: Membrane; Single-pass type I membrane protein: P13746; Secreted : P61769
• Total number of polymer chains: 6
• Total formula weight: 89186.82

Authors

Thong, S.Y.,Yap, J.W.,Lim, P.Y.,Verhelst, S.H.,Lescar, J.,Meijers, R.,Grotenbreg, G.M. (deposition date: 2014-06-19, release date: 2014-09-17, Last modification date: 2024-11-13)

Primary citation

Choo, J.A.L.,Thong, S.Y.,Yap, J.,Van Esch, W.J.E.,Raida, M.,Meijers, R.,Lescar, J.,Verhelst, S.H.L.,Grotenbreg, G.M.
Bioorthogonal Cleavage and Exchange of Major Histocompatibility Complex Ligands by Employing Azobenzene-Containing Peptides.
Angew.Chem.Int.Ed.Engl., 53:13390-, 2014

PubMed Abstract: Bioorthogonal cleavable linkers are attractive building blocks for compounds that can be manipulated to study biological and cellular processes. Sodium dithionite sensitive azobenzene-containing (Abc) peptides were applied for the temporary stabilization of recombinant MHC complexes, which can then be employed to generate libraries of MHC tetramers after exchange with a novel epitope. This technology represents an important tool for high-throughput studies of disease-specific T cell responses.

PubMed: 25348595
DOI: 10.1002/ANIE.201406295

Experimental method

X-RAY DIFFRACTION (2.43 Å)