4ULW

Crystal structure of the ROQ-domain of human ROQUIN1

Summary for 4ULW

Entry DOI	10.2210/pdb4ulw/pdb
Descriptor	ROQUIN-1 (2 entities in total)
Functional Keywords	rna-binding protein, rna binding protein
Biological source	HOMO SAPIENS (HUMAN)
Cellular location	Cytoplasm, P-body : Q5TC82
Total number of polymer chains	2
Total formula weight	34493.69
Authors	Schuetz, A.,Heinemann, U. (deposition date: 2014-05-14, release date: 2015-01-14, Last modification date: 2024-05-08)
Primary citation	Schuetz, A.,Murakawa, Y.,Rosenbaum, E.,Landthaler, M.,Heinemann, U. Roquin Binding to Target Mrnas Involves a Winged Helix-Turn- Helix Motif. Nat.Commun., 5:5701-, 2014 Cited by PubMed Abstract: Roquin proteins mediate mRNA deadenylation by recognizing a conserved class of stem-loop RNA degradation motifs via their Roquin domain. Here we present the crystal structure of a Roquin domain, revealing a mostly helical protein fold bearing a winged helix-turn-helix motif. By combining structural, biochemical and mutation analyses, we gain insight into the mode of RNA binding. We show that the winged helix-turn-helix motif is involved in the binding of constitutive decay elements-containing stem-loop mRNAs. Moreover, we provide biochemical evidence that Roquin proteins are additionally able to bind to duplex RNA and have the potential to be functional in different oligomeric states. PubMed: 25504471 DOI: 10.1038/NCOMMS6701 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.91 Å)

Structure validation