4UJ4

Crystal structure of human Rab11-Rabin8-FIP3

4UJ4 の概要

• エントリーDOI: 10.2210/pdb4uj4/pdb
• 関連するPDBエントリー: 4UJ3 4UJ5
• 分子名称: Ras-related protein Rab-11A, Rab-3A-interacting protein, Rab11 family-interacting protein 3, ... (5 entities in total)
• 機能のキーワード: transport protein, ciliary targeting complex, cilium, vesicular transport, membrane trafficking, rabin8, rab11, fip3
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 203966.95

構造登録者

Vetter, M.,Lorentzen, E. (登録日: 2015-04-08, 公開日: 2015-08-12, 最終更新日: 2024-01-10)

主引用文献

Vetter, M.,Stehle, R.,Basquin, C.,Lorentzen, E.
Structure of Rab11-FIP3-Rabin8 reveals simultaneous binding of FIP3 and Rabin8 effectors to Rab11.
Nat. Struct. Mol. Biol., 22:695-702, 2015

PubMed Abstract: The small GTPase Rab11 and its effectors FIP3 and Rabin8 are essential to membrane-trafficking pathways required for cytokinesis and ciliogenesis. Although effector binding is generally assumed to be sequential and mutually exclusive, we show that Rab11 can simultaneously bind FIP3 and Rabin8. We determined crystal structures of human Rab11-GMPPNP-Rabin8 and Rab11-GMPPNP-FIP3-Rabin8. The structures reveal that the C-terminal domain of Rabin8 adopts a previously undescribed fold that interacts with Rab11 at an unusual effector-binding site neighboring the canonical FIP3-binding site. We show that Rab11-GMPPNP-FIP3-Rabin8 is more stable than Rab11-GMPPNP-Rabin8, owing to direct interaction between Rabin8 and FIP3 within the dual effector-bound complex. The data allow us to propose a model for how membrane-targeting complexes assemble at the trans-Golgi network and recycling endosomes, through multiple weak interactions that create high-avidity complexes.

PubMed: 26258637
DOI: 10.1038/nsmb.3065

実験手法

X-RAY DIFFRACTION (4.2 Å)