4UG0

STRUCTURE OF THE HUMAN 80S RIBOSOME

これはPDB形式変換不可エントリーです。

4UG0 の概要

• エントリーDOI: 10.2210/pdb4ug0/pdb
• EMDBエントリー: 2938
• 分子名称: 28S ribosomal RNA, 60S RIBOSOMAL PROTEIN L7A, 60S RIBOSOMAL PROTEIN L9, ... (83 entities in total)
• 機能のキーワード: ribosome
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• タンパク質・核酸の鎖数: 81
• 化学式量合計: 3835516.96

構造登録者

Khatter, H.,Myasnikov, A.G.,Natchiar, S.K.,Klaholz, B.P. (登録日: 2015-03-20, 公開日: 2015-06-10, 最終更新日: 2019-12-18)

主引用文献

Khatter, K.,Mysanikov, A.G.,Natchiar, S.K.,Klaholz, B.P.
Structure of the human 80S ribosome
NATURE, 520:640-645, 2015

PubMed Abstract: Ribosomes are translational machineries that catalyse protein synthesis. Ribosome structures from various species are known at the atomic level, but obtaining the structure of the human ribosome has remained a challenge; efforts to address this would be highly relevant with regard to human diseases. Here we report the near-atomic structure of the human ribosome derived from high-resolution single-particle cryo-electron microscopy and atomic model building. The structure has an average resolution of 3.6 Å, reaching 2.9 Å resolution in the most stable regions. It provides unprecedented insights into ribosomal RNA entities and amino acid side chains, notably of the transfer RNA binding sites and specific molecular interactions with the exit site tRNA. It reveals atomic details of the subunit interface, which is seen to remodel strongly upon rotational movements of the ribosomal subunits. Furthermore, the structure paves the way for analysing antibiotic side effects and diseases associated with deregulated protein synthesis.

PubMed: 25901680
DOI: 10.1038/nature14427

実験手法

ELECTRON MICROSCOPY (3.6 Å)