4UFC

Crystal structure of the GH95 enzyme BACOVA_03438

4UFC の概要

• エントリーDOI: 10.2210/pdb4ufc/pdb
• 分子名称: GH95, CALCIUM ION, beta-L-galactopyranose, ... (4 entities in total)
• 機能のキーワード: hydrolase, glycoside hydrolase, xylan, alpha-l-galactosidase, gut microbiota
• 由来する生物種: BACTEROIDES OVATUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 183845.09

構造登録者

Rogowski, A.,Briggs, J.A.,Mortimer, J.C.,Tryfona, T.,Terrapon, N.,Lowe, E.C.,Basle, A.,Morland, C.,Day, A.M.,Zheng, H.,Rogers, T.E.,Thompson, P.,Hawkins, A.R.,Yadav, M.P.,Henrissat, B.,Martens, E.C.,Dupree, P.,Gilbert, H.J.,Bolam, D.N. (登録日: 2015-03-16, 公開日: 2015-07-08, 最終更新日: 2023-12-20)

主引用文献

Rogowski, A.,Briggs, J.A.,Mortimer, J.C.,Tryfona, T.,Terrapon, N.,Lowe, E.C.,Basle, A.,Morland, C.,Day, A.M.,Zheng, H.,Rogers, T.E.,Thompson, P.,Hawkins, A.R.,Yadav, M.P.,Henrissat, B.,Martens, E.C.,Dupree, P.,Gilbert, H.J.,Bolam, D.N.
Glycan Complexity Dictates Microbial Resource Allocation in the Large Intestine.
Nat.Commun., 6:7481-, 2015

PubMed Abstract: The structure of the human gut microbiota is controlled primarily through the degradation of complex dietary carbohydrates, but the extent to which carbohydrate breakdown products are shared between members of the microbiota is unclear. We show here, using xylan as a model, that sharing the breakdown products of complex carbohydrates by key members of the microbiota, such as Bacteroides ovatus, is dependent on the complexity of the target glycan. Characterization of the extensive xylan degrading apparatus expressed by B. ovatus reveals that the breakdown of the polysaccharide by the human gut microbiota is significantly more complex than previous models suggested, which were based on the deconstruction of xylans containing limited monosaccharide side chains. Our report presents a highly complex and dynamic xylan degrading apparatus that is fine-tuned to recognize the different forms of the polysaccharide presented to the human gut microbiota.

PubMed: 26112186
DOI: 10.1038/NCOMMS8481

実験手法

X-RAY DIFFRACTION (2.81 Å)