4U3E

Anaerobic ribonucleotide reductase

4U3E の概要

• エントリーDOI: 10.2210/pdb4u3e/pdb
• 分子名称: Ribonucleoside triphosphate reductase, ZINC ION, CITRIC ACID, ... (7 entities in total)
• 機能のキーワード: proteolysis, barrel, reductase, radical, oxidoreductase
• 由来する生物種: Thermotoga maritima
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 158442.46

構造登録者

Funk, M.A.,Drennan, C.L. (登録日: 2014-07-20, 公開日: 2014-09-03, 最終更新日: 2024-10-23)

主引用文献

Wei, Y.,Funk, M.A.,Rosado, L.A.,Baek, J.,Drennan, C.L.,Stubbe, J.
The class III ribonucleotide reductase from Neisseria bacilliformis can utilize thioredoxin as a reductant.
Proc.Natl.Acad.Sci.USA, 111:E3756-E3765, 2014

PubMed Abstract: The class III anaerobic ribonucleotide reductases (RNRs) studied to date couple the reduction of ribonucleotides to deoxynucleotides with the oxidation of formate to CO2. Here we report the cloning and heterologous expression of the Neisseria bacilliformis class III RNR and show that it can catalyze nucleotide reduction using the ubiquitous thioredoxin/thioredoxin reductase/NADPH system. We present a structural model based on a crystal structure of the homologous Thermotoga maritima class III RNR, showing its architecture and the position of conserved residues in the active site. Phylogenetic studies suggest that this form of class III RNR is present in bacteria and archaea that carry out diverse types of anaerobic metabolism.

PubMed: 25157154
DOI: 10.1073/pnas.1414396111

実験手法

X-RAY DIFFRACTION (1.64 Å)