4U2N

Crystal structure of a complex of the Miz1- and Nac1 POZ domains.

4U2N の概要

• エントリーDOI: 10.2210/pdb4u2n/pdb
• 関連するPDBエントリー: 4U2M
• 分子名称: Zinc finger and BTB domain-containing protein 17,Nucleus accumbens-associated protein 1 (2 entities in total)
• 機能のキーワード: poz domain, btb domain, transcription
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus: Q96RE7
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 55721.49

構造登録者

Stead, M.A.,Wright, S.C. (登録日: 2014-07-17, 公開日: 2014-12-10, 最終更新日: 2023-12-20)

主引用文献

Stead, M.A.,Wright, S.C.
Structures of heterodimeric POZ domains of Miz1/BCL6 and Miz1/NAC1.
Acta Crystallogr.,Sect.F, 70:1591-1596, 2014

PubMed Abstract: The POZ domain is an evolutionarily conserved protein-protein interaction domain that is found in approximately 40 mammalian transcription factors. POZ domains mediate both homodimerization and the heteromeric interactions of different POZ-domain transcription factors with each other. Miz1 is a POZ-domain transcription factor that regulates cell-cycle arrest and DNA-damage responses. The activities of Miz1 are altered by its interaction with the POZ-domain transcriptional repressors BCL6 and NAC1, and these interactions have been implicated in tumourigenesis in B-cell lymphomas and in ovarian serous carcinomas that overexpress BCL6 and NAC1, respectively. A strategy for the purification of tethered POZ domains that form forced heterodimers is described, and crystal structures of the heterodimeric POZ domains of Miz1/BCL6 and of Miz1/NAC1 are reported. These structures will be relevant for the design of therapeutics that target POZ-domain interaction interfaces.

PubMed: 25484205
DOI: 10.1107/S2053230X14023449

実験手法

X-RAY DIFFRACTION (2.3 Å)