4U2C

Crystal structure of dienelactone hydrolase A-6 variant (S7T, A24V, Q35H, F38L, Q110L, C123S, Y145C, E199G and S208G) at 1.95 A resolution

Summary for 4U2C

Related4U2B 4U2D 4U2E 4U2F 4U2G
DescriptorCarboxymethylenebutenolidase, SULFATE ION (3 entities in total)
Functional Keywordshydrolase, a/b hydrolase fold
Biological sourcePseudomonas sp.
Total number of polymer chains1
Total molecular weight25378.68
Authors
Porter, J.L.,Collyer, C.A.,Ollis, D.L. (deposition date: 2014-07-16, release date: 2014-12-10, Last modification date: 2020-01-01)
Primary citation
Porter, J.L.,Boon, P.L.,Murray, T.P.,Huber, T.,Collyer, C.A.,Ollis, D.L.
Directed evolution of new and improved enzyme functions using an evolutionary intermediate and multidirectional search.
Acs Chem.Biol., 10:611-621, 2015
PubMed: 25419863 (PDB entries with the same primary citation)
DOI: 10.1021/cb500809f
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.95 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.21500 0.6% 2.6%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload
170172
PDB entries from 2020-10-21