4TSM

MBP-fusion protein of PilA1 from C. difficile R20291 residues 26-166

4TSM の概要

• エントリーDOI: 10.2210/pdb4tsm/pdb
• 関連するPDBエントリー: 4OGM 4PE2
• 関連するBIRD辞書のPRD_ID: PRD_900010
• 分子名称: maltose-binding protein, pilin chimera, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, trimethylamine oxide, ... (4 entities in total)
• 機能のキーワード: pilin, t4p, fusion, cell adhesion
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 172568.36

構造登録者

Piepenbrink, K.H.,Sundberg, E.J. (登録日: 2014-06-19, 公開日: 2015-01-14, 最終更新日: 2023-09-27)

主引用文献

Piepenbrink, K.H.,Maldarelli, G.A.,Martinez de la Pena, C.F.,Dingle, T.C.,Mulvey, G.L.,Lee, A.,von Rosenvinge, E.,Armstrong, G.D.,Donnenberg, M.S.,Sundberg, E.J.
Structural and Evolutionary Analyses Show Unique Stabilization Strategies in the Type IV Pili of Clostridium difficile.
Structure, 23:385-396, 2015

PubMed Abstract: Type IV pili are produced by many pathogenic Gram-negative bacteria and are important for processes as diverse as twitching motility, biofilm formation, cellular adhesion, and horizontal gene transfer. However, many Gram-positive species, including Clostridium difficile, also produce type IV pili. Here, we identify the major subunit of the type IV pili of C. difficile, PilA1, and describe multiple 3D structures of PilA1, demonstrating the diversity found in three strains of C. difficile. We also model the incorporation of both PilA1 and a minor pilin, PilJ, into the pilus fiber. Although PilA1 contains no cysteine residues, and therefore cannot form the disulfide bonds found in all Gram-negative type IV pilins, it adopts unique strategies to achieve a typical pilin fold. The structures of PilA1 and PilJ exhibit similarities with the type IVb pilins from Gram-negative bacteria that suggest that the type IV pili of C. difficile are involved in microcolony formation.

PubMed: 25599642
DOI: 10.1016/j.str.2014.11.018

実験手法

X-RAY DIFFRACTION (1.899 Å)