4TNT

Structure of the human mineralocorticoid receptor in complex with DNA

4TNT の概要

• エントリーDOI: 10.2210/pdb4tnt/pdb
• 分子名称: Mineralocorticoid receptor, DNA (5'-D(*CP*AP*GP*AP*AP*CP*AP*CP*TP*CP*TP*GP*TP*TP*CP*TP*G)-3'), DNA (5'-D(*CP*AP*GP*AP*AP*CP*AP*GP*AP*GP*TP*GP*TP*TP*CP*TP*G)-3'), ... (5 entities in total)
• 機能のキーワード: dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 33247.25

構造登録者

Hudson, W.H.,Ortlund, E.A. (登録日: 2014-06-04, 公開日: 2014-09-17, 最終更新日: 2023-12-27)

主引用文献

Hudson, W.H.,Youn, C.,Ortlund, E.A.
Crystal structure of the mineralocorticoid receptor DNA binding domain in complex with DNA.
Plos One, 9:e107000-e107000, 2014

PubMed Abstract: The steroid hormone receptors regulate important physiological functions such as reproduction, metabolism, immunity, and electrolyte balance. Mutations within steroid receptors result in endocrine disorders and can often drive cancer formation and progression. Despite the conserved three-dimensional structure shared among members of the steroid receptor family and their overlapping DNA binding preference, activation of individual steroid receptors drive unique effects on gene expression. Here, we present the first structure of the human mineralocorticoid receptor DNA binding domain, in complex with a canonical DNA response element. The overall structure is similar to the glucocorticoid receptor DNA binding domain, but small changes in the mode of DNA binding and lever arm conformation may begin to explain the differential effects on gene regulation by the mineralocorticoid and glucocorticoid receptors. In addition, we explore the structural effects of mineralocorticoid receptor DNA binding domain mutations found in type I pseudohypoaldosteronism and multiple types of cancer.

PubMed: 25188500
DOI: 10.1371/journal.pone.0107000

実験手法

X-RAY DIFFRACTION (2.3938 Å)