4TNO

Hypothetical protein PF1117 from Pyrococcus Furiosus: Structure solved by sulfur-SAD using Swiss Light Source Data

Summary for 4TNO

• Entry DOI: 10.2210/pdb4tno/pdb
• Related: 2I0X
• Descriptor: CRISPR-associated endoribonuclease Cas2, CHLORIDE ION (3 entities in total)
• Functional Keywords: sulfur sad, hydrolase
• Biological source: Pyrococcus furiosus
• Total number of polymer chains: 1
• Total formula weight: 10063.58

Authors

Weinert, T.,Waltersperger, S.,Olieric, V.,Panepucci, E.,Chen, L.,Rose, J.P.,Wang, M.,Wang, B.C.,Southeast Collaboratory for Structural Genomics (SECSG) (deposition date: 2014-06-04, release date: 2014-12-10, Last modification date: 2023-12-27)

Primary citation

Weinert, T.,Olieric, V.,Waltersperger, S.,Panepucci, E.,Chen, L.,Zhang, H.,Zhou, D.,Rose, J.,Ebihara, A.,Kuramitsu, S.,Li, D.,Howe, N.,Schnapp, G.,Pautsch, A.,Bargsten, K.,Prota, A.E.,Surana, P.,Kottur, J.,Nair, D.T.,Basilico, F.,Cecatiello, V.,Pasqualato, S.,Boland, A.,Weichenrieder, O.,Wang, B.C.,Steinmetz, M.O.,Caffrey, M.,Wang, M.
Fast native-SAD phasing for routine macromolecular structure determination.
Nat.Methods, 12:131-133, 2015

PubMed Abstract: We describe a data collection method that uses a single crystal to solve X-ray structures by native SAD (single-wavelength anomalous diffraction). We solved the structures of 11 real-life examples, including a human membrane protein, a protein-DNA complex and a 266-kDa multiprotein-ligand complex, using this method. The data collection strategy is suitable for routine structure determination and can be implemented at most macromolecular crystallography synchrotron beamlines.

PubMed: 25506719
DOI: 10.1038/nmeth.3211

Experimental method

X-RAY DIFFRACTION (2.14 Å)