4TNN

Crystal structure of Escherichia coli protein YodA in complex with Ni - artifact of purification.

Summary for 4TNN

DescriptorMetal-binding lipocalin, SULFATE ION, NICKEL (II) ION, ... (4 entities in total)
Functional Keywordsyoda, purification artifact, metal-binding lipocalin, metal binding protein
Biological sourceEscherichia coli str. K-12 substr. MC4100
Total number of polymer chains1
Total molecular weight23008.95
Authors
Gasiorowska, O.A.,Cymborowski, M.T.,Handing, K.B.,Shabalin, I.G.,Zasadzinska, E.,Niedzialkowska, E.,Porebski, P.J.,Minor, W. (deposition date: 2014-06-04, release date: 2014-06-25, Last modification date: 2019-12-25)
Primary citation
Niedzialkowska, E.,Gasiorowska, O.,Handing, K.B.,Majorek, K.A.,Porebski, P.J.,Shabalin, I.G.,Zasadzinska, E.,Cymborowski, M.,Minor, W.
Protein purification and crystallization artifacts: The tale usually not told.
Protein Sci., 25:720-733, 2016
PubMed: 26660914 (PDB entries with the same primary citation)
DOI: 10.1002/pro.2861
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.951 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.214101.2%1.0%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload