1.8 A resolution room temperature structure of Thermolysin recorded using an XFEL

Summary for 4TNL

DescriptorThermolysin, ZINC ION, CALCIUM ION, ... (4 entities in total)
Functional Keywordszn protease, x-ray free electron laser, hydrolase
Biological sourceBacillus thermoproteolyticus
Cellular locationSecreted P00800
Total number of polymer chains1
Total molecular weight34586.06
Primary citation
Kern, J.,Tran, R.,Alonso-Mori, R.,Koroidov, S.,Echols, N.,Hattne, J.,Ibrahim, M.,Gul, S.,Laksmono, H.,Sierra, R.G.,Gildea, R.J.,Han, G.,Hellmich, J.,Lassalle-Kaiser, B.,Chatterjee, R.,Brewster, A.S.,Stan, C.A.,Glockner, C.,Lampe, A.,DiFiore, D.,Milathianaki, D.,Fry, A.R.,Seibert, M.M.,Koglin, J.E.,Gallo, E.,Uhlig, J.,Sokaras, D.,Weng, T.C.,Zwart, P.H.,Skinner, D.E.,Bogan, M.J.,Messerschmidt, M.,Glatzel, P.,Williams, G.J.,Boutet, S.,Adams, P.D.,Zouni, A.,Messinger, J.,Sauter, N.K.,Bergmann, U.,Yano, J.,Yachandra, V.K.
Taking snapshots of photosynthetic water oxidation using femtosecond X-ray diffraction and spectroscopy.
Nat Commun, 5:4371-4371, 2014
PubMed: 25006873 (PDB entries with the same primary citation)
DOI: 10.1038/ncomms5371
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.234200.4%1.6%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload