4TNH

RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution

Summary for 4TNH

• Entry DOI: 10.2210/pdb4tnh/pdb
• Related: 4TNI 4TNJ 4TNK
• Descriptor: Photosystem Q(B) protein 1, Photosystem II reaction center protein K, Photosystem II reaction center protein L, ... (35 entities in total)
• Functional Keywords: photosynthesis, water oxidation, membrane protein, x-ray free electron laser, electron transport
• Biological source: Thermosynechococcus elongatus; More
• Total number of polymer chains: 40
• Total formula weight: 762724.63

Authors

Kern, J.,Tran, R.,Alonso-Mori, R.,Koroidov, S.,Echols, N.,Hattne, J.,Ibrahim, M.,Gul, S.,Laksmono, H.,Sierra, R.G.,Gildea, R.J.,Han, G.,Hellmich, J.,Lassalle-Kaiser, B.,Chatterjee, R.,Brewster, A.,Stan, C.A.,Gloeckner, C.,Lampe, A.,DiFiore, D.,Milathianaki, D.,Fry, A.R.,Seibert, M.M.,Koglin, J.E.,Gallo, E.,Uhlig, J.,Sokaras, D.,Weng, T.-C.,Zwart, P.H.,Skinner, D.E.,Bogan, M.J.,Messerschmidt, M.,Glatzel, P.,Williams, G.J.,Boutet, S.,Adams, P.D.,Zouni, A.,Messinger, J.,Sauter, N.K.,Bergmann, U.,Yano, J.,Yachandra, V.K. (deposition date: 2014-06-04, release date: 2014-07-09, Last modification date: 2024-10-23)

Primary citation

Kern, J.,Tran, R.,Alonso-Mori, R.,Koroidov, S.,Echols, N.,Hattne, J.,Ibrahim, M.,Gul, S.,Laksmono, H.,Sierra, R.G.,Gildea, R.J.,Han, G.,Hellmich, J.,Lassalle-Kaiser, B.,Chatterjee, R.,Brewster, A.S.,Stan, C.A.,Glockner, C.,Lampe, A.,DiFiore, D.,Milathianaki, D.,Fry, A.R.,Seibert, M.M.,Koglin, J.E.,Gallo, E.,Uhlig, J.,Sokaras, D.,Weng, T.C.,Zwart, P.H.,Skinner, D.E.,Bogan, M.J.,Messerschmidt, M.,Glatzel, P.,Williams, G.J.,Boutet, S.,Adams, P.D.,Zouni, A.,Messinger, J.,Sauter, N.K.,Bergmann, U.,Yano, J.,Yachandra, V.K.
Taking snapshots of photosynthetic water oxidation using femtosecond X-ray diffraction and spectroscopy.
Nat Commun, 5:4371-4371, 2014

PubMed Abstract: The dioxygen we breathe is formed by light-induced oxidation of water in photosystem II. O2 formation takes place at a catalytic manganese cluster within milliseconds after the photosystem II reaction centre is excited by three single-turnover flashes. Here we present combined X-ray emission spectra and diffraction data of 2-flash (2F) and 3-flash (3F) photosystem II samples, and of a transient 3F' state (250 μs after the third flash), collected under functional conditions using an X-ray free electron laser. The spectra show that the initial O-O bond formation, coupled to Mn reduction, does not yet occur within 250 μs after the third flash. Diffraction data of all states studied exhibit an anomalous scattering signal from Mn but show no significant structural changes at the present resolution of 4.5 Å. This study represents the initial frames in a molecular movie of the structural changes during the catalytic reaction in photosystem II.

PubMed: 25006873
DOI: 10.1038/ncomms5371

Experimental method

X-RAY DIFFRACTION (4.90000680874 Å)