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4RX0

Crystal structure of Plasmodium falciparum dihydroorotate dehydrogenase bound with Inhibitor DSM265

Summary for 4RX0
Entry DOI10.2210/pdb4rx0/pdb
DescriptorDihydroorotate dehydrogenase (quinone), mitochondrial, 2-(1,1-difluoroethyl)-5-methyl-N-[4-(pentafluoro-lambda~6~-sulfanyl)phenyl][1,2,4]triazolo[1,5-a]pyrimidin-7-amine, FLAVIN MONONUCLEOTIDE, ... (6 entities in total)
Functional Keywordsalpha/beta barrel, oxidoreductase, fm, mitochondrial membrane, oxidoreductase-oxidoreductase inhibitor complex, oxidoreductase/oxidoreductase inhibitor
Biological sourcePlasmodium falciparum
Total number of polymer chains1
Total formula weight46505.78
Authors
Deng, X.,Phillips, M.,Tomchick, D. (deposition date: 2014-12-08, release date: 2015-07-29, Last modification date: 2023-09-20)
Primary citationPhillips, M.A.,Lotharius, J.,Marsh, K.,White, J.,Dayan, A.,White, K.L.,Njoroge, J.W.,El Mazouni, F.,Lao, Y.,Kokkonda, S.,Tomchick, D.R.,Deng, X.,Laird, T.,Bhatia, S.N.,March, S.,Ng, C.L.,Fidock, D.A.,Wittlin, S.,Lafuente-Monasterio, M.,Benito, F.J.,Alonso, L.M.,Martinez, M.S.,Jimenez-Diaz, M.B.,Bazaga, S.F.,Angulo-Barturen, I.,Haselden, J.N.,Louttit, J.,Cui, Y.,Sridhar, A.,Zeeman, A.M.,Kocken, C.,Sauerwein, R.,Dechering, K.,Avery, V.M.,Duffy, S.,Delves, M.,Sinden, R.,Ruecker, A.,Wickham, K.S.,Rochford, R.,Gahagen, J.,Iyer, L.,Riccio, E.,Mirsalis, J.,Bathhurst, I.,Rueckle, T.,Ding, X.,Campo, B.,Leroy, D.,Rogers, M.J.,Rathod, P.K.,Burrows, J.N.,Charman, S.A.
A long-duration dihydroorotate dehydrogenase inhibitor (DSM265) for prevention and treatment of malaria.
Sci Transl Med, 7:296ra111-296ra111, 2015
Cited by
PubMed Abstract: Malaria is one of the most significant causes of childhood mortality, but disease control efforts are threatened by resistance of the Plasmodium parasite to current therapies. Continued progress in combating malaria requires development of new, easy to administer drug combinations with broad-ranging activity against all manifestations of the disease. DSM265, a triazolopyrimidine-based inhibitor of the pyrimidine biosynthetic enzyme dihydroorotate dehydrogenase (DHODH), is the first DHODH inhibitor to reach clinical development for treatment of malaria. We describe studies profiling the biological activity, pharmacological and pharmacokinetic properties, and safety of DSM265, which supported its advancement to human trials. DSM265 is highly selective toward DHODH of the malaria parasite Plasmodium, efficacious against both blood and liver stages of P. falciparum, and active against drug-resistant parasite isolates. Favorable pharmacokinetic properties of DSM265 are predicted to provide therapeutic concentrations for more than 8 days after a single oral dose in the range of 200 to 400 mg. DSM265 was well tolerated in repeat-dose and cardiovascular safety studies in mice and dogs, was not mutagenic, and was inactive against panels of human enzymes/receptors. The excellent safety profile, blood- and liver-stage activity, and predicted long half-life in humans position DSM265 as a new potential drug combination partner for either single-dose treatment or once-weekly chemoprevention. DSM265 has advantages over current treatment options that are dosed daily or are inactive against the parasite liver stage.
PubMed: 26180101
DOI: 10.1126/scitranslmed.aaa6645
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.25 Å)
Structure validation

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