Crystal structure of a pentafluoro-Phe incorporated Phosphatidylinositol-specific phospholipase C (H258X)from Staphylococcus aureus

Summary for 4RV3

Descriptor1-phosphatidylinositol phosphodiesterase, ACETATE ION, 1,2,3,4,5,6-HEXAHYDROXY-CYCLOHEXANE, ... (4 entities in total)
Functional Keywordscation-pi, tim barrel, encoded unnatural amino acid phospholipase, lyase
Biological sourceStaphylococcus aureus
Total number of polymer chains1
Total molecular weight34535.26
He, T.,Gershenson, A.,Eyles, S.J.,Gao, J.,Roberts, M.F. (deposition date: 2014-11-24, release date: 2015-07-01, Last modification date: 2018-08-29)
Primary citation
He, T.,Gershenson, A.,Eyles, S.J.,Lee, Y.J.,Liu, W.R.,Wang, J.,Gao, J.,Roberts, M.F.
Fluorinated Aromatic Amino Acids Distinguish Cation-pi Interactions from Membrane Insertion.
J.Biol.Chem., 290:19334-19342, 2015
PubMed: 26092728 (PDB entries with the same primary citation)
DOI: 10.1074/jbc.M115.668343
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.240200.4%0.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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