Crystal structure of Y-family DNA polymerase Dpo4 bypassing a MeFapy-dG adduct

Summary for 4RUA

Related4RU9 4RUC
DescriptorDNA polymerase IV, Nucleic acids Template: TCAT(MF7)GAATCCTTCCCCC, Nucleic acids Primar: GGGGGAAGGATTC, ... (6 entities in total)
Functional Keywords2, protein, dna, dna damage, dna-directed dna polymerase, adenosine triphosphate, y-family polymerase, trans-lesion synthesis (tls), dna binding, mefapy-dg lesion bypass, 6-diamino-4-hydroxy n(5)-(methyl)-formamidopyrimidine (mefapy-dg) lesion, transferase-dna complex, transferase/dna
Biological sourceSulfolobus solfataricus P2
Cellular locationCytoplasm  Q97W02
Total number of polymer chains3
Total molecular weight49147.14
Patra, A.,Banerjee, S.,Stone, M.P.,Egli, M. (deposition date: 2014-11-18, release date: 2015-08-05)
Primary citation
Patra, A.,Banerjee, S.,Johnson Salyard, T.L.,Malik, C.K.,Christov, P.P.,Rizzo, C.J.,Stone, M.P.,Egli, M.
Structural Basis for Error-Free Bypass of the 5-N-Methylformamidopyrimidine-dG Lesion by Human DNA Polymerase eta and Sulfolobus solfataricus P2 Polymerase IV.
J.Am.Chem.Soc., 137:7011-7014, 2015
PubMed: 25988947 (PDB entries with the same primary citation)
DOI: 10.1021/jacs.5b02701
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.25341.8%4.3%0.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 4rua
no rotation
Molmil generated image of 4rua
rotated about x axis by 90°
Molmil generated image of 4rua
rotated about y axis by 90°